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Generating high-quality genome drafts from uncultured microbiome samples with a single shotgun experiment
This webinar will feature a presentation on a recently published method for de novo assembly of microbial genome drafts using short read sequencing. The method uses Chromium Linked-Read sequencing and a novel computational analysis pipeline, Athena, to construct high-quality microbial genome drafts of metagenomic communities sampled directly from their environments. In uncultured samples from human stool, Athena produced individual genome drafts with high contiguity (>200-kb N50, fewer than ten contigs), even for bacteria with relatively low raw short-read sequence coverage. Compared with results from existing short-read and synthetic long-read metagenomic sequencing techniques, the Athena assemblies are the most contiguous reported to date.

Speaker:
Dr. Ami Bhatt, MD, PhD
Assistant Professor of Medicine and Genetics
Stanford University

Feb 13, 2019 9:00 AM in Pacific Time (US and Canada)

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